Anti-PCOLCE Antibody Picoband® A06428-2 from BosterBio

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Anti-PCOLCE Antibody Picoband®

Western blot analysis of PCOLCE using anti-PCOLCE antibody (A06428-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCOLCE antigen affinity purified polyclonal antibody (Catalog # A06428-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCOLCE at approximately 50 kDa. The expected band size for PCOLCE is at 48 kDa.
Anti-PCOLCE Antibody Picoband®

IHC analysis of PCOLCE using anti-PCOLCE antibody (A06428-2).
PCOLCE was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCOLCE Antibody (A06428-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Anti-PCOLCE Antibody Picoband®

IHC analysis of PCOLCE using anti-PCOLCE antibody (A06428-2).
PCOLCE was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCOLCE Antibody (A06428-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-PCOLCE Antibody Picoband®

BosterBio's Anti-PCOLCE Antibody Picoband® is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: EIA, Immunoassay, ELISA, Flow Cytometry, Immunohistochemistry, and Western Blot. The Anti-PCOLCE Antibody Picoband® was generated using PCOLCE as the antigen and it reacts with Homo Sapiens, and Human.

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Boster Bio Anti-PCOLCE Antibody Picoband™ catalog # A06428-2. Tested in ELISA, IHC, WB, Flow Cytometry (Intracellular) applications. This antibody reacts with Human

Anti-PCOLCE Antibody Picoband®

Anti-PCOLCE Antibody Picoband®

Anti-PCOLCE Antibody Picoband®

Anti-PCOLCE Antibody Picoband® from BosterBio

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BosterBio

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Anti-PCOLCE Antibody Picoband® from BosterBio

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